目的目前修复骨缺损的方式中,自体骨供量有限,异体骨或异种骨移植常出现术后排异反应,针对牙槽骨缺损修复而设计出一种来源广、价格低且生物相容性佳的骨组织工程材料迫在眉睫。文章旨在构建还原氧化石墨烯(rGO)修饰的壳聚糖骨组织工程支架,评价其表面形貌、理化特性及生物学性能。方法通过化学还原法制备rGO,利用扫描电镜(SEM)、透射电镜及傅里叶红外光谱对其表征。再利用冷冻干燥法制备出CS/GP、0.1%rGO/CS/GP、0.25%rGO/CS/GP和0.5%rGO/CS/GP四组复合支架,SEM下观察支架表面形貌;计算各组支架的孔径、孔隙率、吸水率和降解率;电子万能试验机进行压缩强度测试,筛选出修饰支架的合适rGO浓度后,MTS检测两组支架细胞增殖速率,激光共聚焦观察两组支架鬼笔环肽染色。结果TEM下可更明显地观察到rGO的薄层结构,呈半透明状态,选区电子衍射图可见明显的六角衍射光斑。CS/GP组支架的网状骨架上较为平整;0.1%rGO/CS/GP组支架的个别网状骨架上可见rGO片层附着其上,但是数量较少,不易发现;0.25%rGO/CS/GP组支架的大多数网状骨架上均可观察到rGO片层的附着,使大多数的网状骨架上均呈现出褶皱状态;0.5%rGO/CS/GP组支架则可以发现很多的网状结构内有多数rGO片层堆叠,堵塞住许多原本的孔状结构。0.5%rGO/CS/GP组支架的孔隙率比CS/GP、0.25%rGO/CS/GP组低(P<0.05)。0.1%、0.25%、0.5%rGO/CS/GP吸水率(535.87%±13.21%、621.48%±15.99%、637.19%±11.73%)均高于CS/GP复合支架(343.98%±11.03%),差异具有统计学意义(P<0.05)。CS/GP复合支架的降解速率最快,在第7和21天时分别达到46.47%±3.13%和81.35%±0.85%,均高于0.1%、0.25%、0.5%rGO/CS/GP组支架(P<0.05)。复合支架的弹性模量也随着支架内rGO含量的增加而相应增加[CS/GP:(1.10±0.14)MPa;0.1%rGO/CS/GP:(1.24±0.21)MPa;0.25%rGO/CS/GP:(1.47±0.21)MPa;0.5%rGO/CS/GP:(1.87±0.25)MPa],其中0.5%rGO/CS/GP复�Objective The aim was to synthesize chitosan/β-glycerophosphate scaffolds modified by reduced graphene oxide(rGO)and evaluate its surface morphology and physical and chemical properties.Methods rGO was synthesized by a chemical reduction method and then was characterized by scanning electron microscope(SEM),transmission electron microscope(TEM),and Fourier infrared spectroscopy(FTIR).After the chitosan/β-glycerophosphate(CS/GP)dispersion,the composite scaffolds of different concentrations rGO were prepared via freeze-drying technology.SEM was used to observe its surface morphology.The pores,porosities,water absorption,and degradations of the scaffolds were investigated by SEM,liquid displacement method,and gravimetric method.The mechanical property of the scaffolds was analyzed by the compression test.The cell proliferation rate of two groups was tested by MTS,and the cell adhesion was observed by phalloidin staining.Results rGO had a wrinkled sheet structure,and most of the oxygen-containing groups on the surface were removed.0.25%rGO/CS/GP scaffolds under SEM showed that the rGO sheet was uniformly bound to the trabecular surface of the porous in CS/GP scaffolds.Its pore was 113.19±46.85μm,porosity was 52.99±6.75%,water absorption was 621.48±15.99%,and the degradation rate of 21 days was 64.72±2.24%.Strain can reach to 0.29 MPa when its stress was 50%,and elastic modulus was 1.47±0.21 MPa.The cell proliferation rate on the 5th and 7th day was higher than CS/GP scaffolds.Conclusion The rGO is successfully prepared by chemical reduction.rGO is successfully modified into the CS/GP scaffold.Scaffolds modified by 0.25%rGO can improve mechanical properties,increase the water absorption rate,reduce the degradation rate,and promote the adhesion and proliferation of hDPSCs.
机构地区南方医科大学南京临床医学院（东部战区总医院）口腔科
出处《医学研究生学报》 CAS 北大核心 2021年第4期350-356,共7页Journal of Medical Postgraduates
基金国家自然科学基金(82071094,81700990)。
关键词壳聚糖 还原氧化石墨烯 骨组织工程chitosan reduced graphene oxide bone tissue engineering
分类号Q819 [生物学—生物工程]
