不同产地白英中澳洲茄胺的含量测定

摘要

目的:建立HPLC测定白英中澳洲茄胺含量的方法。方法:采用Agilent Zorabax-SB-C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-水为流动相,梯度洗脱,流速1.0 mL/min,检测波长为210 nm。结果:澳洲茄胺含量在0.4μg~1.8μg范围内有良好的线性关系,计算得回归方程Y=4791.6X-230(r2=0.9993),平均加样回收率为101.4%(RSD=2.2%)。13个不同产地的白英药材中,澳洲茄胺的含量在2.0 mg/g~2.4 mg/g之间。结论:所建立的HPLC法精密度、稳定性和重复性均良好,可用于白英中澳洲茄胺的含量测定。Objective:To establish an HPLC method for the determination of solasodine in Solanum lyratum Thunb.Method:Agilent SB ODS C18 columm(250 mm×4.6 mm,5μm)was used and the detection wavelength was 210 nm.The mobile phase consisted of acetonitrile-water with flow rate of 1.0 mL/min.Result:The linear range of solasodine was 0.4~1.8μg(r=0.9996)and the average recovery rate was 101.4%(RSD=2.2%).Regression equation Y=4791.6 X-230(r2=0.9993).The content of solaniline in Solanum lyratum Thunb from 13 different areas ranged from 2.0 mg/g to 2.4 mg/g.Conclusion:The method has good precision,stability and repeatability,and could be used to control the quantity of Solanum lyratum Thunb.

作者梁锐高太祥赵峰李珊珊王瑞Liang Rui;Gao Taixiang;Zhao Feng;Li Shanshan;Wang Rui(Shanxi Univresity of Chinese Medicine,Jinzhong Shanxi 030619)

机构地区山西中医药大学

出处《山西中医药大学学报》 2021年第1期33-35,共3页Journal of Shanxi University of Chinese Medicine

基金山西省食品药品监督管理局科研项目(2012002A) 晋药综合开发利用协同创新中心项目(2017-JYXT-15)。

关键词白英高效液相色谱含量测定澳洲茄胺Solanum lyratum Thunb HPLC content determination solasodine

分类号R284.2 [医药卫生—中药学]