摘要 花生果腐病是花生生产上的一种重要病害,对花生的产量和品质构成了严重威胁。本研究以花育20号感病前后籽仁为样品,进行转录组测序,得到Unigene 60756个,其中表达量上调的基因10147个,下调的基因4334个;GO富集分析表明,差异表达基因被分类到24种细胞组分、5种分子功能和23种生物过程中;KEGG富集分析发现有4291个差异基因被注释到34条代谢通路中。筛选得到多条花生响应果腐病相关的激素信号转导途径,通过分析相关基因的表达图谱,推测花生可能通过JA/ET介导的抗病途径来抵抗果腐病病原菌的侵染。 Pod rot,an important disease affecting peanut production,causes serious damage to peanut yield and quality.In this study,RNA sequencing analysis was performed with peanut cultivar Huayu20 kernels that infected with pod rot disease in field,and that uninfected kernels acting as control.As a result,a total of 60,756 unigenes were obtained,among which the expression of 10,147 genes were up-regulated and 4,334 genes were down-regulated.Further,Gene ontology enrichment analysis showed that the differentially expressed genes(DEGs)were assigned to 24 cellular components,5 molecular functions and 23 biological processes.Through Kyoto Encyclopedia of Genes and Genomes enrichment analysis,a total of 4,291 DEGs were mapped to 34 metabolic pathways.We identified multiple hormone signal transduction pathways that were responsive to pod rot disease infection.based on the expression pattern of related genes,we speculate that peanuts may resist pod rot disease infection through jasmonic acid-and ethylene-mediated pathways.
出处 《花生学报》 北大核心 2021年第1期1-11,共11页 Journal of Peanut Science
基金 国家自然科学基金项目(31701464) 国家花生产业技术体系项目(CARS-13) 泰山学者工程专项(tsnq201812121) 山东省自然科学基金项目(ZR2020MC103) 山东省农业科学院农业科技创新工程(CXGC2016B02,CXGC2018E21) 山东省重大科技创新工程项目(2019JZZY010702) 广东省重点领域研发计划项目(2020B020219003) 青岛市科技惠民示范引导专项(20-3-4-26-nsh) 临沂市重点研发计划(2019YD009)。
关键词 花生 果腐病 转录组测序 JA/ET信号转导途径 peanut pod rot disease transcriptome JA and ET signal transduction pathway
