摘要 为探究水盐共同作用下花生根际土壤微生物的响应,从花生根际细菌群落结构的角度阐述干旱和盐胁迫对花生根际细菌菌群多样性的影响及与根际微生物间的相互作用。采用盆栽试验,设计对照、盐胁迫、中度干旱胁迫和旱盐同时胁迫四个处理,通过16S rRNA基因测序技术,研究花生饱果期根际土壤细菌群落结构和变化情况。结果表明:干旱胁迫、盐胁迫及二者同时胁迫处理的优势菌门均为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)、绿弯菌门(Chloroflexi)和拟杆菌门(Gemmatimonadota)。干旱胁迫和盐胁迫均使得变形菌门和酸杆菌门相对丰度降低,而蓝藻菌门(Cyanobacteria)的相对丰度提高,但在干旱和盐同时胁迫条件下蓝藻菌门的相对丰度降幅达60.0%。本试验为特异功能物种和菌株的分离提供了更大的可能和空间,为促生菌的分离培养优化和应用提供了技术基础和前景。非生物胁迫影响根际土壤微域环境,对花生根际土壤细菌群落结构具有调控作用。调节微生物群落结构,改良土壤微域环境,是提高植物胁迫耐受性的有效途径。 To explore the response of peanut rhizosphere soil bacteria to water and salt stress,the effects of drought and salt stress on the diversity of peanut rhizosphere bacterial community and their interaction with rhizosphere bacteria were elaborated from the perspective of peanut rhizosphere bacterial community structure.A pot experiment was conducted with four treatments:control,salt stress,moderate drought stress,and simultaneous drought and salt stress.The structure and variation of the bacterial community in peanut rhizosphere soil during the pod-filling stage were studied with 16S rRNA gene sequencing technology.The results showed that the dominant phyla under drought stress,salt stress,and double stress were Proteobacteria,Actinobacteria,Acidobacteria,Chloroflexi,and Gemmatimonadates.The relative abundance of Proteobacteria and Acidobacteria decreased,while that of Cyanobacteria increased under either drought or salt stress.However,the abundance value of Cyanobacteria decreased by 60.0%under simultaneous drought and salt stress.This experiment provided a greater possibility and space for the isolation of specific functional bacterial species and strains,and provided technical basis and prospect for the isolation,culture optimization,and application of growth-promoting bacteria.Abiotic stress affects the rhizosphere soil microenvironment and regulates the bacterial community structure of peanut rhizosphere soil.Modifying microbial community structure and enhancing soil microenvironment are effective ways to improve plant stress tolerance.
出处 《花生学报》 北大核心 2021年第1期33-40,共8页 Journal of Peanut Science
基金 国家自然科学基金面上项目(31971856) 国家重点研发计划项目(2018YFD0201000) 山东省现代农业产业技术体系创新团队(花生)项目(SDAIT-04-06) 山东省农业科学院创新工程项目(CXGC2018B05) 山东省重大科技创新工程项目(2019JZZY010702)。
