摘要 为了对生产群中的无特定病原体(specific pathogen free,SPF)级近交系小鼠进行遗传质量检测,以确认其品系正确,未发生突变。选取2个品系(Balb/cByJ和C57BL/6J)雌雄各半,共16只小鼠,通过多重靶向多聚酶链反应(PCR),扩增包含112个单核苷酸多态性(single nucleotide polymorphism,SNP)位点的DNA片段,对其进行二代测序(next-generation sequencing,NGS),获得它们在这些SNP位点的基因型。经二代测序后,得到有效读取数为6118873,每个位点的平均读取数为3631,97个SNP位点在所有的小鼠中都被分型,另外15个SNP位点,至少在1个样本中没有被检出。其中,6个SNP只在一个品系的小鼠中有结果,还有2个SNP只在一种性别的小鼠中有结果。在所有样本中得到的全部SNP分型结果都符合其品系的基因型,且均为纯合子。送检的小鼠遗传特征稳定,利用二代测序技术进行SNP分型,能对近交系小鼠的遗传特性进行快速准确的评估。 To detect the genetic quality of SPF inbred mice from proliferation population,as well as to make sure the absence of mutation and the conformation of strains,eight mice each from two strains(Balb/cByJ and C57BL/6J)with half male and half female were genotyped for 112 single nucleotide polymorphism(SNP)loci by the method of multiplex targeting PCR combined with next-generation sequencing.The results showed that 6118873 effective reads in all were obtained by next-generation sequencing(NGS),and the average number of reads for each SNP was 3631,97 SNPs were genotyped successfully in all mice,while the other 15 failed in being detected in at least one mouse.Among which,six SNPs could be genotyped in only one strain of mice,and two other SNPs had results only in male mice.The genotyping results for all of the samples agreed with the genotype of their respective strains,and all the tested loci were homozygous.The tested mice had stable genetic characteristics.The genotyping for specific SNPs by NGS can evaluate the genetic characteristics of inbred strains of mice with high efficiency and accuracy.
机构地区 新疆医科大学动物实验中心
出处 《动物医学进展》 北大核心 2021年第5期62-66,共5页 Progress In Veterinary Medicine
基金 新疆维吾尔自治区自然科学基金项目(2018D01C178)。
关键词 近交系小鼠 单核苷酸多态性 多重靶向聚合酶链式反应 二代测序 inbred strain mice single nucleotide polymorphism multiplex targeting PCR next-generation sequencing
