摘要 为了解种子颜色目测法和色差计法对小麦穗发芽抗性的鉴定效果,利用这两种方法对1018份小麦材料种子颜色进行测定,并分析了色差计量指标L*、a*、b*及a*/b*、b*/a*、a*/L*、b*/L*值与种子颜色和穗发芽抗性的关系。结果表明:(1)红粒小麦a*、a*/b*和a*/L*的均值均显著高于白粒小麦,其他指标均值显著低于白粒小麦;(2)a*/b*和b*/a*值与种子颜色相关性强,相关系数分别为-0.934和0.941,其线性回归方程可用于判断小麦种子颜色;(3)红粒小麦的穗发芽抗性显著高于白粒小麦,但不同红粒品种之间穗发芽抗性差异很大,变异系数为122.36%;(4)种子颜色及a*/b*和b*/a*值与穗发芽抗性相关性强,相关系数分别为0.854、-0.831和0.835;其次是a*/L*值与穗发芽抗性的相关性,其相关系数为-0.735;(5)目测法获得的种子颜色可解释穗发芽抗性72.9%的变异,色差计参数可解释穗发芽抗性72.5%的变异,但在单独分析时,色差计参数对红粒和白粒小麦穗发芽抗性分别仅能解释11.4%和8.7%的变异。总之,色差计法测量种子颜色,可以作为目测法判别小麦种子颜色的补充方法,用于种子颜色定性和定量比较分析。尽管红粒小麦比白粒小麦抗穗发芽,但鉴于红粒小麦品种(系)间穗发芽抗性差异很大,仍需进行穗发芽抗性鉴定。 In order to understand the effect of visual method and colorimeter for identification of resistance to pre-harvest sprouting,the grain colors of 1018 wheat materials were determined with the two methods,and the relationship between color measurement indexes including L*,a*,b*,a*/b*,b*/a*,a*/L*and b*/L*were analyzed.The results showed that:the mean values of a*,a*/b*and a*/L*of red-grained wheat were significantly higher than those of white-grained wheat,and the average values of other indexes were significantly lower than those of white-grained wheat.a*/b*and b*/a*values had strong correlation with grain color,and the correlation coefficients were-0.934 and 0.941,respectively.Grain color,a*/b*and b*/a*had strong correlation with pre-harvest sprouting resistance,and the correlation coefficients were 0.854,-0.831 and 0.835,respectively.The correlation coefficient between a*/L*value and pre-harvest sprouting resistance was-0.735.The grain color obtained by visual method can explain 72.9%of the variation of pre-harvest sprouting resistance.The colorimeter method can explain 72.5%of the variation of pre-harvest sprouting resistance,but only 11.4%and 8.7%of the variation of red-grained and white-grained wheat can be explained by colorimeter method,respectively.To conclude,the colorimeter method can be used as a supplementary method to visual method to determine the color of wheat grain,which can be used for qualitative and quantitative comparative analysis of grain color.Although red-grained wheat is more resistant to pre-harvest germination than white-grained wheat,it is still necessary to identify the pre-harvest sprouting resistance in view of the great difference in pre-harvest sprouting resistance among red-grained wheat varieties.
出处 《麦类作物学报》 CAS CSCD 北大核心 2021年第4期424-429,共6页 Journal of Triticeae Crops
基金 国家重点研发计划项目(2017YFD0100802,2016YFD0101802) 国家小麦产业技术体系项目(CARS-03) 湖北省技术创新专项重大项目(2018ABA085) 粮食作物种质创新与遗传改良湖北省重点实验室开放课题(2017lzjj02)。
