摘要 目的探讨γ干扰素(IFN?γ)+874位点(rs2430561)单核苷酸多态性及IFN?γ血清水平与带状疱疹易感性的相关性。方法于南部战区总医院及海南省第五人民医院收集74例带状疱疹患者血液样本,40例健康对照血液样本。采用PCR重测序法检测受试者rs2430561位点单核苷酸多态性,荧光定量PCR检测受试者血清水痘带状疱疹病毒DNA拷贝数,酶联免疫吸附试验检测血清IFN?γ水平。计量资料的比较采用t检验或非参数检验,计数资料的比较采用卡方检验或Fisher确切概率法。结果带状疱疹组rs2430561位点基因型分别为AA型4例、TT型37例和TA型33例,健康对照组分别为TA型13例、TT型27例,带状疱疹组A等位基因比例(27.70%)高于健康对照组(16.25%,P=0.036)。带状疱疹组血清IFN?γ水平M(P25,P75)为[33.45(0.80,95.01)pg/ml],低于健康对照组[67.83(2.74,318.35)pg/ml,U=1822,P=0.028]。带状疱疹组血清水痘带状疱疹病毒DNA拷贝数(lg值)为(3.23±0.71)/ml,血清IFN?γ水平与水痘带状疱疹病毒DNA拷贝数呈负相关(r=-0.302,P=0.009)。结论携带rs2430561位点A等位基因可能影响IFN?γ的表达,导致对带状疱疹病毒有更高的易感性。 Objective To investigate the correlation of interferon(IFN)-γrs2430561 single nucleotide polymorphisms(SNPs)and serum IFN-γlevels with susceptibility to herpes zoster.Methods Blood samples were collected from 74 patients with herpes zoster and 40 healthy controls in General Hospital of Southern Theatre Command and the Fifth People Hospital of Hainan Province from November 2019 to November 2020.PCR and Sagner resequencing were conducted to detect the IFN-γrs2430561 SNPs in the subjects,fluorescence-based quantitative PCR was performed to determine the copy number of varicella-herpes zoster virus(VZV)DNA in the serum of the patients with herpes zoster,and enzyme-linked immunosorbent assay was conducted to detect the serum IFN-γlevel.Measurement data were compared by using t test or non-parametric test,and enumeration data by using chi-square test or Fisher′s exact test.Results As rs2430561 genotyping showed,there were 4 patients with AA genotype,37 with TT genotype and 33 with TA genotype in the herpes zoster group,as well as 13 subjects with TA genotype and 27 with TT genotype in the healthy control group,and the frequency of A allele of rs2430561 was significantly higher in the herpes zoster group(27.70%)than in the control group(16.25%,P=0.036).The serum IFN-γlevel(M[P25,P75])was significantly lower in the herpes zoster group(33.45[0.80,95.01]pg/ml)than in the control group(67.83[2.74,318.35]pg/ml,U=1822,P=0.028).The VZV DNA copy number(expressed as a logarithm to the base of 10)per milliliter was 3.23±0.71 in the serum of the patients with herpes zoster,and the serum IFN-γlevel was negatively correlated with the VZV DNA copy number(r=-0.302,P=0.009).Conclusion The carriage of rs2430561 A allele may affect the expression of IFN-γ,leading to higher susceptibility to herpes zoster.
出处 《中华皮肤科杂志》 CAS CSCD 北大核心 2021年第6期510-513,共4页 Chinese Journal of Dermatology
基金 国家自然科学基金(81673072)。
关键词 带状疱疹 多态性 单核苷酸 干扰素Γ rs2430561位点 Herpes zoster Polymorphism single nucleotide Interferon-gamma rs2430561
