《缺氧环境对鼻咽纤维血管瘤细胞增殖、侵袭的影响及可能的作用机制》为作者：陆徐最新的研究成果，本论文的主要观点为目的：探讨缺氧环境下培养鼻咽血管纤维瘤（nasopharyngeal angiofibroma，NA）细胞的生物学功能变化以及可能的分子机制。方法：将NA细胞置于不同浓度的氧气环境（20%、1%O2）中培养，CCK-8法检测NA细胞的增殖活性，Transwell检测NA细胞的侵袭能力，QPCR或western blot检测NA细胞中HIF-1α、VEGF、TGF-β、ERα的表达。结果：与常氧状态相比，缺氧状态下的NA细胞增殖活性增强（P<0。001），侵袭能力显著上升（P<0。001），HIF-1α、VEGF、TGF-β表达均上调，ERα表达下调（P均<0。05）。结论：缺氧环境下，NA细胞可能通过上调HIF-1α、VEGF、TGF-β表达，下调ERα表达，来增强细胞的增殖、侵袭能力，促进NA进展。 关键词：缺氧；鼻炎纤维血管瘤；HIF-1α Abstract: Objective: To investigate the changes of biological function and possible molecular mechanism of nasopharyngeal angiofibroma (NA) cells cultured in hypoxia environment。 Methods: NA cells were cultured in different concentrations of oxygen (20%, 1% O2)。 The proliferative activity of NA cells was detected by CCK-8 method, the invasiveness of NA cells was detected by Transwell, and the expression of HIF-1 α, VEGF, TGF-β, ERα in NA cells was detected by qPCR or Western blot。 Results: compared with the normoxia state, the proliferation activity of NA cells in hypoxia state was enhanced (P < 0。001), the invasion ability was significantly increased (P < 0。001), the expression of HIF-1 α, VEGF, TGF-β was up-regulated, and the expression of ERα was down regulated (P<0。05)。 Conclusion: under hypoxia environment, Na cells may enhance the proliferation and invasion of cells and promote the progress of Na by up regulating the expression of HIF-1α, VEGF, TGF-β and down regulating the expression of ERα。 Keywords: hypoxia; rhinitis fibroangioma; HIF-1α。不知是否符合录用要求，望您批评与指正。