《不同剂量维生素C对脓毒症诱发小鼠急性肺损伤的影响》为作者：葛晨最新的研究成果，本论文的主要观点为摘要】目的 探讨不同剂量维生素C对脓毒症诱发小鼠急性肺损伤的影响。方法 清洁级雄性昆明小鼠100只，体重约35g，按随机数字表随机分为5组：假手术组（n=20）、脓毒症组（n=20）、大剂量维生素C组（维生素C 1组，200mg/kg， n=20）、中等剂量维生素C组（维生素C 2组，100mg/kg， n=20）、常规剂量维生素C组（维生素C 3组，50mg/kg， n=20），假手术组仅进行开腹探查盲肠，脓毒症组采用盲肠结扎穿孔术（CLP），维生素C 1组、2组、3组分别在CLP后3h尾静脉注射200mg/kg、100mg/kg、50mg/kg维生素C。分别于术后48h采用酶联免疫法（ELISA）测定各组肺组织炎症因子、氧化应激指标含量，采用流式细胞法测定各组肺组织细胞凋亡率，并于光镜下观察肺组织病理学结果。 结果 与假手术组比较，脓毒症组术后48h小鼠病死率、肺组织上清液炎症因子及氧化应激产物明显升高，肺组织细胞凋亡率升高（P均<0。05），肺损伤明显加重；与脓毒症组比较，维生素C 1组、2组术后48h小鼠病死率、肺组织上清液炎症因子及氧化应激产物降低，肺组织细胞凋亡率下降（P均<0。05），肺损伤减轻；与脓毒症组比较，维生素C 3组术后48h小鼠病死率、肺组织上清液炎症因子及氧化应激产物无明显变化（P>0。05）。结论 相对大剂量维生素C对脓毒症诱发的小鼠肺损伤有保护作用。【Abstract】Objective To evaluate the effect of diffrent doses of ascorbic acid on acute lung injury mice induced by sepsis。 Methods Qne hundred healthy male Kunming mice, about weighing 35g, were randomly divided into 5 groups: sham operation group (n=20), sepsis group (n=20), high dose of ascorbic acid group (ascorbic acid group 1, n=20), middle dose of ascorbic acid group (ascorbic acid group 2, n=20) and normal dose of ascorbic acid group (ascorbic acid group 3, n=20)。 Sham group were exposed to the cecum only。 Sepsis was induced by CLP。 In ascorbic acid group 1, 2 and 3, 200mg/kg, 100mg/kg and 50mg/kg ascorbic acid were injected into the candal vein 3 hours after CLP in mice。 The mice were sacrificed and the lung samples were collected at 48h after operation for determination of inflammatory factor (by ELISA), oxidative stress index (by ELISA), lung tissue cells apoptosis rate (by flow cytometry) and for microscopic examination。 Results Compared with sham group, the inflammatory factor, oxidative stress index and lung tissue cells apoptosis rate were higher than those in sepsis group。 And the microscopic examination showed that lung injury was aggravated in sepsis group。 Compared with sepsis group, the inflammatory factor, oxidative stress index and lung tissue cells apoptosis rate were lower than those in ascorbic acid group 1 and 2。 And the microscopic examination was attenuated in ascorbic acid group 1 and 2。 Compared with sepsis group, various indexes showed no significant change in ascorbic acid group 3。 Conclusion Relative high dose of ascorbic acid was related to protect of acute lung injury in sepsis in mice。不知是否符合录用要求，望您批评与指正。