摘要 目的探讨不同类型的抗血管生成靶向治疗对紫杉醇致大鼠神经病理性疼痛程度的影响。方法将32只成年雄性SD大鼠随机分为空白对照组、对照组、恩度组及安维汀组,每组8只。对照组、恩度组及安维汀组大鼠分别于第1 d、3 d、5 d、7 d腹腔注射2.0 mg/(kg·d)紫杉醇。恩度组大鼠于第1 d、3 d、5 d、7 d通过尾静脉注射10 mg/(kg·d)恩度,对照组、安维汀组大鼠于相同时间点注入相同体积的生理盐水;安维汀组大鼠于第1 d腹腔注射20 mg/(kg·d)安维汀,对照组、恩度组大鼠于相同时间点腹腔注射相同体积的生理盐水。于实验前1 d和第3 d、5 d、7 d、12 d、16 d、20 d测定大鼠的机械缩足反射阈值(MWT)。采用透射电镜观察大鼠坐骨神经的超微结构,用免疫组化法检测大鼠坐骨神经中血管内皮生长因子(VEGF)的表达。结果空白对照组、对照组、恩度组、安维汀组大鼠实验前后肢MWT差异无统计学意义(均P>0.05)。与空白对照组比较,对照组、恩度组、安维汀组大鼠第3 d、5 d、7 d、12 d、16 d、20 d后肢MWT显著降低(均P<0.05)。恩度组、安维汀组大鼠第5 d、16 d后肢MWT显著高于对照组(均P<0.05)。透射电镜下可见对照组大鼠坐骨神经损伤最严重;恩度组、安维汀组坐骨神经损伤较对照组轻,其中恩度组更轻一些。恩度组、安维汀组大鼠VEGF表达显著低于对照组(均P<0.05)。结论抗血管生成靶向治疗对紫杉醇所致的大鼠神经病理性疼痛的程度影响不明显,但对坐骨神经有一定的保护作用。 Objective To investigate the effect of different types of antiangiogenic targeted therapy on paclitaxel-induced neuropathic pain in rats.Methods Thirty-two adult male SD rats were randomly divided into blank control group,control group,Endostatin group,Avastin group,8 rats in each group.The rats in the control group,Endostatin group and Avastin group were intraperitoneally injected with paclitaxel 2.0 mg/(kg·d)at 1 d,3 d,5 d,7 d after treatment.The rats in the Endostatin group were given Endostatin 10 mg/(kg·d)at 1 d,3 d,5 d,7 d after treatment by tail vein injection,and the rats in the control group,Avastin group were given the same volume of saline by tail vein injection at same time points.The rats in the Avastin group were given Avastin 20 mg/(kg·d)by peritoneal injection,the rats in the control group,Endostatin group were given the same volume of saline by peritoneal injection at the same time points.Mechanical withdrawal threshold(MWT)of rats hind paw were measured 1 d before and 3 d,5 d,7 d,12 d,16 d,20 d after treatment.The sciatic nerve was analyzed by an electron microscope,and immunohistochemical method was used to detect the expressions of vascular endothelial growth factor(VEGF)protein.Results The difference of MWT of posterior limb in the blank control group,control group,Endostatin group,Avastin group was no statistical significance before treatment(all P>0.05).Compared with those in the blank control group,the MWT of posterior limb in the control group,Endostatin group and Avastin group at 3 d,5 d,7 d,12 d,16 d,20 d after treatment were significantly decreased(all P<0.05).The MWT of posterior limb in Endostatin group,Avastin group at 5 d,16 d after treatment were significantly increased than those in control group(all P<0.05).The injury of the sciatic nerve was most serious in the control group;the injury in the Endostatin group,Avastin group was slighter than that in control group,and the injury were the slightest in the Endostatin group.The expression of VEGF in the sciatic nerve in the Endos
机构地区 武汉市第一医院肿瘤科
出处 《临床神经病学杂志》 CAS 2021年第1期41-45,共5页 Journal of Clinical Neurology
基金 武汉市卫健委科研项目(WX19D02)。
关键词 抗血管生成靶向治疗 紫杉醇 神经病理性疼痛 血管内皮生长因子 antiangiogenic targeted therapy paclitaxel neuropathic pain vascular endothelial growth factor
