摘要 通过四组平行小试装置,研究空床水力停留时间(HRT)对缺氧好氧生物滤池(Anoxic oxic biofilter,A/O-BF)去除低碳氮比(C/N)农村生活污水中氮的影响;结合高通量测序,研究了不同HRT条件下的脱氮效能和微生物群落变化特征,优选出最佳HRT.结果表明:①缺氧段HRT为6 h的条件下,好氧段HRT为9和10 h时,A/O-BF对NH4+-N和TN的平均去除率均分别为99%和75%,显著高于HRT为7 h(分别为93%和66%)和8 h(分别为96%和70%)时的情况.②好氧段HRT为9 h的条件下,缺氧段HRT为6 h时,A/O-BF对TN的平均去除率为78%,明显高于HRT为4 h(59%)、稍高于HRT为5 h(74%)和7 h(75%)时的情况.③好氧段HRT为9 h时,其硝化菌属Nitrosomonas和Nitrospira的总丰度占11.42%,明显高于HRT为7 h(4.61%)、8 h(7.39%)和10 h(7.64%)时的情况.④缺氧段HRT为6 h时,其主要的反硝化菌属Denitratisoma占5.83%,明显高于HRT为4 h(0.81%)、5 h(2.90%)和7 h(3.27%)时的情况.研究显示,HRT影响A/O-BF的脱氮效能和微生物群落分布特征,处理低碳氮比(1.4~3.0)农村生活污水时,A/O-BF好氧段和缺氧段的最佳HRT分别为9和6 h. Four sets of bench-scale anoxic oxic biofilters(A/O-BF)were set up to study the influence of hydraulic retention time(HRT)on the nitrogen removal from simulated low carbon-to-nitrogen(C/N)ratio rural domestic sewage.Combined with the high-throughput sequencing method,the characteristics of nitrogen removal efficiency and microbial communities were extensively investigated at different HRTs,and the best HRT for the anoxic and oxic column of A/O-BF was recommended.The results were as follows:(1)When the HRT of anoxic column maintained at 6 h,the average NH4+-N and TN removal efficiency of A/O-BF was 99%and 75%at 9 h and 10 h HRT in the oxic column,which was significantly higher than those at 7 h(93%and 66%)and 8 h(96%and 70%)HRT.(2)When the HRT of the oxic column was 9 h,the TN removal efficiency of A/O-BF was 78%at 6 h HRT in the anoxic column,which was significantly higher than that at 4 h(59%)and slightly higher than that at 5 h(74%)and 7 h(75%)HRT.(3)The total proportion of Nitrosomonas and Nitrospira in the oxic column at 9 h(11.42%)HRT was significantly higher than that at 7 h(4.61%),8 h(7.39%)and 10 h(7.64%)HRT.The proportion of the major denitrifying bacteria,i.e.Denitratisoma in the anoxic column at 6 h(5.83%)HRT was significantly higher than that at 4 h(0.81%),5 h(2.90%)and 7 h(3.27%)HRT.(4)The nitrogen removal efficiency and microbial community distribution of A/O-BF were influenced by the HRT,and 9 h and 6 h were the optimum HRT for the oxic and anoxic column of A/O-BF during the treatment of rural domestic sewage with low C/N ratio.
出处 《环境科学研究》 CAS CSCD 北大核心 2021年第4期927-935,共9页 Research of Environmental Sciences
基金 中央级公益性科研院所基本科研业务专项(No.2016YSKY-031) 国家水体污染控制与治理科技重大专项(No.2017ZX07401003-05-01)。
关键词 缺氧好氧生物滤池(A/O-BF) 水力停留时间(HRT) 低碳氮比(C/N) 农村生活污水 脱氮 anoxic oxic biofilters(A/O-BF) hydraulic retention time(HRT) low carbon-to-nitrogen(C/N)ratio rural domestic sewage nitrogen removal
