摘要 【目的】通过对簇毛麦与7182杂交获得的抗条锈病新种质V832进行抗条锈病鉴定和遗传分析,明确V832含有的抗病基因以及细胞学特性.【方法】以V832、感病对照铭贤169及其杂交后代F 1、F 2、F 3和BC 1群体为材料,采用当前流行的条锈菌生理小种(菌系)Su11-4、Su11-7、CYR23、CYR29、CYR32、CYR33和CYR34对供试群体进行苗期抗条锈性鉴定,分析抗病基因的遗传规律,并利用基因组原位杂交技术对V832含有的外源染色体片段进行鉴定.【结果】V832在苗期对7个条锈菌生理小种均表现免疫或近免疫,其抗性可能来源于簇毛麦.V832对Su11-7和CYR32的抗病性都是由一对显性基因控制.GISH分析表明V832含有来自簇毛麦的染色体片段,是一个普通小麦-簇毛麦易位系.【结论】簇毛麦易位系V832对我国目前流行的小麦条锈菌生理小种具有良好的抗病性,可以作为抗源在我国小麦抗条锈育种中应用. 【Objective】To study the inheritance of resistance to stripe rust in V832,a wheat-Haynaldia villosa germplasm,V832 was developed from the interspecific hybridization between wheat genotype 7182 and Haynaldia villosa.【Method】Seven different races of Puccinia striiformis f.sp.tritici(Pst),Su11-4,Su11-7,CYR23,CYR29,CYR32,CYR33 and CYR34,were selected to test the F1,F2,F2:3,BC1 progenies derived from V832/Mingxian 169 in seedling stage in greenhouse.Genomic in situ hybridization(GISH)were applied to identify H.villosa chromosomes in V832.【Result】V832 is resistant to all tested stripe rust races.Genetic analysis revealed that the resistance to Su11-7 and CYR32 was controlled by a single dominant gene respectively.The translocation alien chromatin in V832 was obvious and cytologically detectable,further indicating that V832 is a translocation line.【Conclusion】This study indicated that V832 had a considerable application potential in wheat breeding for rust resistance.
机构地区 长江大学农学院 四川省农业科学院植物保护研究所
出处 《甘肃农业大学学报》 CAS CSCD 北大核心 2021年第1期37-41,共5页 Journal of Gansu Agricultural University
基金 国家重点研发计划项目(2018YFD0200506) 国家自然科学基金项目(31501620).
