摘要 【目的】认识高温胁迫条件下不同玉米材料间的基因差异表达,为阐明玉米耐高温胁迫的分子遗传机制提供理论基础.【方法】以耐高温胁迫的中地88和高温敏感的先玉335两个玉米品种为试验材料,在42℃高温条件下取其花粉提取总RNA,再经mRNA纯化、反转录、文库构建及转录组测序等一系列过程,对其基因差异表达作生物信息学分析.【结果】共发现基因24553个,以先玉335为对照,上调表达基因857个,下调表达基因965个,无显著差异表达基因22731个,这些基因较均一地分布于玉米的10条染色体.对差异表达基因进行GO富集分析,结果显示:与细胞组分、分子功能及生物过程相关的GO条目数分别为491、1062和2320个;在GO注释的差异表达基因中,进一步分析发现直接应答于热激效应的基因有7个,其中2个上调表达,5个下调表达;与热激蛋白结合有关的基因有3个,其中1个上调表达,2个下调表达.对差异表达基因作KEGG富集分析发现有102个小通路,这些小通路可进一步归为6个大通路,包括细胞过程3个、环境信息处理4个、遗传信息处理20个、人类疾病1个、新陈代谢72个和有机系统2个.【结论】转录组测序是在基因组水平认识基因差异表达的有效方法,共发现12个与玉米耐高温胁迫相关的差异表达基因. 【Objective】To realize differential expression of genes between different maize(Zea mays L.)materials,and to provide some theoretical basis for elucidating the molecular genetic mechanism of tolerance to high temperature stress in maize.【Method】Two maize varieties,Zhongdi 88(resistant to high temperature stress)and Xianyu 335(sensitive to high temperature stress),were used as experimental materials,and their pollen was taken under high temperature of 42℃for extracting total RNA,after a series of processes including mRNA purification,reverse transcription,library construction and transcriptome sequencing,the differential expression of genes were analyzed by means of bioinformatics.【Result】Total of 24553 genes were found.Taking Xianyu 335 as the control,857 up-regulated genes,965 down-regulated genes,and 22731 undifferentiated expressed genes were found,these genes were evenly distributed on ten chromosomes.GO enrichment analysis of differentially expressed genes showed that the number of GO items related to cell components,molecular functions and biological processes were 491,1062 and 2320,respectively.Among the differentially expressed genes by GO annotation,further analysis found that 7 genes were directly responsive to heat shock,among which 2 were up-regulated and 5 were down-regulated;three genes were involved in the binding of heat shock proteins,of which 1 was up-regulated and 2 were down-regulated.The KEGG enrichment analysis of differentially expressed genes revealed 102 pathways,which could be further classified into 6 major pathways,including 3 cellular processes,4 environmental information processing,20 genetic information processing,1 human disease,72 metabolism and 2 organic systems.【Conclusion】Transcriptome sequencing is an effective method to recognize the differential expression of genes at genomic level,a total of 12 differentially expressed genes related to maize resistance to high temperature stress were found in this experiment.
出处 《甘肃农业大学学报》 CAS CSCD 北大核心 2021年第1期42-49,共8页 Journal of Gansu Agricultural University
基金 南充市研发资金项目(19YFZJ0081) 西华师范大学校基本科研业务费(19B033) 山西省农科院特色农业技术项目(YGG1701).
分类号 S513 [农业科学—作物学]
