金针菇子实体多糖FVPB1对小鼠B细胞的免疫调节作用

摘要通过检测金针菇(Flammulina filiformis)子实体多糖FVPB1对小鼠B细胞的体外增殖、激活、分泌免疫球蛋白和白细胞介素-10(IL-10),以及调节IL-10相关信号通路的作用,评价FVPB1的免疫调节活性。结果表明:FVPB1可激活B细胞并促使其增殖、分泌免疫球蛋白IgG、IgM和产生IL-10,且可通过细胞外调节蛋白激酶、c-Jun氨基末端激酶和P38丝裂原活化蛋白激酶相关信号通路激活B细胞释放IL-10。 Heteropolysaccharide FVPB1 was purified from Flammulina velutipes fruiting body and evaluated for its biological activities on proliferation of mouse B cells,activation of spleen lymphocytes,and secretion of IgG,IgM and interleukin-10(IL-10)by mouse B cells in vitro.The results showed that FVPB1 stimulated B cells,promoted B cell proliferation,and increased secretion of IgG,IgM and IL-10 by B cells.FVPB1 activated Breg to release IL-10 via JNK,P38 and ERK1/2 signaling pathways.

作者王慧敏张劲松冯婷包海鹰余养朝冯占贾薇汪雯翰 WANG Huimin;ZHANG Jingsong;FENG Ting;BAO Haiying;YU Yangchao;FENG Zhan;JIA Wei;WANG Wenghan(College of Food Science&Technology,Shanghai Ocean University,Shanghai 201306,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungal Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungal Processing,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China;College of Chinese Traditional Medicine,Jilin Agricultural University,Changchun 130118,Jilin,China;Jiangsu Chinagreen Biotechnology Co.,Ltd.,Suqian 223700,Jiangsu,China)

机构地区上海海洋大学食品学院上海市农业科学院食用菌研究所吉林农业大学中药材学院江苏华绿生物科技股份有限公司

出处《食用菌学报》 CSCD 北大核心 2021年第2期69-76,共8页 Acta Edulis Fungi

基金上海市农业科学院卓越团队[农科创2017(A-06)] 2020年江苏省宿迁市“千名领军人才集聚计划”。

关键词 B细胞免疫球蛋白 IL-10 B cells immunoglobulin IL-10

分类号 R28 [医药卫生—中药学]