梯棱羊肚菌子实体多糖提取优化及结构初探

摘要采用酶辅助法提取梯棱羊肚菌(Morchella importuna)子实体多糖,以粗多糖得率和多糖含量为指标,通过单因素、Plackett-Burman、最陡爬坡和Box-Behnken实验优化出梯棱羊肚菌子实体多糖的提取工艺:料液比为1∶20(W∶V),中性蛋白酶添加量8 mg·g^-1,49.7℃酶解1 h后,80℃浸提4.1 h,提取液脱蛋白后用体积浓度82%乙醇沉淀,在此条件下,粗多糖得率为7.47%,多糖含量为62.18%。优化条件下提取的粗多糖重均分子量为1.51×10⁶ g·mol^-1,单糖组成为葡萄糖、甘露糖和半乳糖,其摩尔比为0.64∶0.55∶0.27。 Using crude polysaccharide yield and polysaccharide content as indicies,enzyme-assisted extraction of polysaccharides from fruiting bodies of Morchella importuna was optimized by single factor experiment,Plackett-Burman design,the steepest ascent experiment,and Box-Behnken design.The results showed that the optimal extraction conditions were as follows:ratio of raw material to water at 1∶20(W∶V),enzymolysis by 8 mg·g^-1 dispase at 49.7℃for 1 h,extraction at 80℃for 4.1 h,and precipitation by 82%ethanol(V∶V)after deproteinization.Using the optimized conditions,the crude polysaccharide yield was 7.47%and the polysaccharide content was 62.18%.The average molecular weight of the crude polysaccharide extract obtained under the optimized conditions was 1.51×10⁶ Da,and its monosaccharides included glucose,mannose,and galactose with a molar ratio of 0.64∶0.55∶0.27.

作者何畅徐丽婧常明昌孟俊龙左宁柯王昭玉 HE Chang;XU Lijing;CHANG Mingchang;MENG Junlong;ZUO Ningke;WANG Zhaoyu(College of Food Science and Engineering,Shanxi Agricultural University,Jinzhong 030801,Shanxi,China;Shanxi Engineering Research Center of Edible Fungi,Jinzhong 030801,Shanxi,China)

机构地区山西农业大学食品科学与工程学院山西省食用菌工程技术研究中心

出处《食用菌学报》 CSCD 北大核心 2021年第2期77-88,共12页 Acta Edulis Fungi

基金山西省重点研发计划重点项目(201703D211001) 山西省应用基础研究项目(201801D221300) 食用菌山西省科技创新重点团队(201805D131009)。

关键词梯棱羊肚菌粗多糖得率多糖含量重均分子量单糖组成 Morchella importuna crude polysaccharide yield polysaccharide content average molecular weight monosaccharide composition

分类号 R28 [医药卫生—中药学]