基于牛血清白蛋白功能化金纳米棒的荧光探针测定Hg2+

摘要建立了一种以牛血清白蛋白功能化的金纳米棒(BSA-Cys-GNRs)为荧光探针检测Hg²⁺的新方法。以半胱氨酸作为连接臂成功将牛血清白蛋白修饰在金纳米棒表面,通过紫外可见吸收光谱、荧光光谱、红外光谱和荧光倒置显微镜等多种分析方法对材料进行表征。研究发现,在295nm波长光激发下,BSA-Cys-GNRs探针在338nm显示强荧光,而Hg²⁺能够有效地猝灭BSA-Cys-GNRs的荧光。对一系列影响猝灭效果的因素进行考察,得出pH 4.0、孵育时间5.0min为最佳检测条件。Mn²⁺、K⁺、Ni²⁺、Na⁺、Cr³⁺、Cd²⁺、Mg²⁺、Cu²⁺、Ca²⁺、Al³⁺和Zn²⁺对BSA-Cys-GNRs的荧光信号没有明显的影响。当Hg²⁺的浓度为0.04444~8.888μmol·L^-1时,荧光猝灭效率与Hg²⁺的浓度之间存在良好的线性关系,检测限为8.08nmol·L^-1。将该方法用于环境水样中Hg²⁺的检测,回收率为98.9%~105.0%。 Bovine serum albumin(BSA)functionalized gold nanorods(BSA-Cys-GNRs)as a fluorescence probe for the detection of Hg²⁺has been developed in this work.BSA was successfully modified on the surface of gold nanorods with cysteine as the linker.The materials were characterized by UV-Vis,FL,FT-IR and inverted fluorescence microscopy.The BSA-Cys-GNRs displays strong fluorescence emission peak at 338 nm under excitation at 295 nm.The fluorescence of BSA-Cys-GNRs is strongly quenched upon addition of Hg²⁺ions.Effective detection conditions are pH 4.0 and incubating time 5.0 min.Mn²⁺,K⁺,Ni²⁺,Na⁺,Cr³⁺,Cd²⁺,Mg²⁺,Cu²⁺,Ca²⁺,Al³⁺and Zn²⁺are examined under identical conditions,and all of them do not induce any noticeable responses to BSA-Cys-GNRs.There is a good linear dependence of fluorescence intensity on Hg²⁺concentration in the range of 0.04444~8.888μmol·L^-1with the detection limit of 8.08 nmol·L^-1.Furthermore,the method has been applied for the determination of Hg²⁺in water samples,and the recoveries are in the range of 98.9%~105.0%.

作者李瑞军朱长龙季一兵 Li Ruijun;Zhu Changlong;Ji Yibing(Department of Analytical Chemistry,School of Science,China Pharmaceutical University,Nanjing,211198)

机构地区中国药科大学理学院分析化学教研室

出处《化学通报》 CAS CSCD 北大核心 2021年第3期261-266,193,共7页 Chemistry

基金国家自然科学基金项目(21804141)资助。

关键词金纳米棒荧光探针 Hg²⁺ Gold nanorods Fluorescent probe Hg²⁺

分类号 R28 [医药卫生—中药学]