摘要 目的通过病毒宏基因组学分析镇江地区上呼吸道病毒感染患儿鼻腔病毒谱构成。方法收集100份呼吸道疾病患儿鼻黏膜分泌物样本,提取核酸,采用高通量测序平台进行测序,通过病毒宏基因组学分析平台分析病毒谱。结果镇江地区上呼吸道病毒感染患儿鼻腔微环境内病毒群落主要由腺病毒科(30.13%)、小RNA病毒科(24.04%)、冠状病毒科(11.22%)、指环病毒科(10.58%)、多瘤病毒科(10.58%)和副黏液病毒科(7.37%)等组成。所检测文库病毒主要为腺病毒科(30.13%)和小RNA病毒科(24.04%)。结论镇江地区上呼吸道病毒感染患儿鼻腔内病毒类型较为丰富,主要为腺病毒科和小RNA病毒科。上呼吸道病毒感染患儿鼻腔病毒谱的初步筛查,可为儿童病毒性上呼吸道感染的预防及临床治疗提供实验室依据。 Objective Viral macrogenomics was used to study the composition of viral communities in nasal cavity of children with upper respiratory tract viral infection in Zhenjiang.Methods A total of 100 nasal mucosal secretion samples were collected from children with upper respiratory tract viral infection.Nucleic acid was extracted and sequenced by Miseque high-throughput sequencing.The viral community was analyzed by viral macrogenomics analysis platform.Results The viral community in the microenvironment of children's nasal mucosal secretion samples in Zhenjiang was mainly composed of adenoviridae(30.13%),picornaviridae(24.04%),coronaviridae(11.22%)and actinoviridae(10.58%),polyomaviridae(10.58%),paramyxoviridae(7.37%)and so on.The detected library viruses were mainly adenoviridae(30.13%)and picornaviridae(24.04%).Conclusions The virus spectrum of children's upper respiratory tract viral infection in Zhenjiang is various,which are mainly adenoviridae and picornaviridae.It provides a reference for the prevention and clinical treatment of children with upper respiratory tract viral infection.
机构地区 江苏大学医学院
出处 《检验医学》 CAS 2021年第4期408-411,共4页 Laboratory Medicine
基金 国家自然科学基金项目(81741062) 江苏省重点研发计划(BE2017693)。
关键词 儿童 上呼吸道 病毒宏基因组学 高通量测序 腺病毒科 小RNA病毒科 镇江地区 Children Upper respiratory tract Viral mecrogenomics High-throughput sequencing Adenoviridae Picornaviridae
