摘要 为探讨姬松茸多糖对高脂血症大鼠降血脂作用及机制,采用水提醇沉法提取姬松茸粗多糖,并用DEAE-纤维素离子交换柱对多糖进行层析分级,采用高效液相色谱法分析单糖组成,并测定多糖含量;高脂饮食诱导建立高脂血症大鼠模型,将大鼠随机分成4组(空白对照组、模型组、辛伐他汀阳性对照组、姬松茸多糖组),同时对大鼠口服灌胃给药,连续42 d;测定大鼠相对肝脏指数,ELISA法检测大鼠血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)含量;HE染色法观察肝脏组织病理变化,RT-PCR法检测大鼠肝脏组织中低密度脂蛋白(LDL-R)、固醇调节原件结合蛋白-1C(SREBP-1C)、胆固醇7a-羟化酶(CYP7a-1)、过氧化物酶体增殖物激活受体α(PPAR-α)、B类清道夫F(SCARB-1F)的mRNA表达水平。研究结果显示:姬松茸多糖中糖的质量分数为75.1%,DEAE-纤维素离子交换柱层析分级得到姬松茸酸性多糖级分(ABP-A),ABP-A中含有质量分数为88.6%的葡萄糖、2.4%的半乳糖、1.9%的甘露糖、1.0%的鼠李糖、0.5%的半乳糖醛酶、1.9%的木糖、2.1%的阿拉伯糖、1.6%的岩藻糖;ABP-A干预后大鼠肝脏指数显著降低(P<0.05);血清TC、TG、LDL-C含量极显著降低(P<0.01),HDL-C含量极显著升高(P<0.01);肝脏内脂滴沉积减少,肝脏中LDL-R、SCARB-1F mRNA相对表达量显著升高(P<0.05),CYP7a-1、PPAR-αmRNA相对表达量极显著升高(P<0.01),SREBP-1C mRNA相对表达量极显著降低(P<0.01)。ABP-A具有显著的降血脂作用,ABP-A的降血脂机制可能与其调控肝脏脂质代谢关键因子LDL-R、SREBP-1C、CYP7a-1、PPAR-α、SCARB-1F的表达有关。 In order to investigate the effect and mechanism of Agaricus blazei Murill polysaccharide on reducing blood lipid in hyperlipidemic rats,the crude polysaccharide was extracted by water extraction and alcohol precipitation method,and the polysaccharide was graded by DEAE cellulose ion exchange column chromatography.The monosaccharide composition was analyzed by high performance liquid chromatography,and the content of polysaccharide was determined.The rats were randomly divided into four groups:blank control group,model group,simvastatin positive control group,Agaricus blazei Murill polysaccharide group,and oral administration for 42 days.The contents of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C)and high density lipoprotein cholesterol(HDL-C)in serum were detected by ELISA.HE staining method was used to observe the pathological changes of liver tissue,and the mRNA levels of LDL-R,SREBP-1C,CYP7a-1,PPAR-αand SCARB-1F were detected by RT-PCR.The results showed that the content of sugar in Agaricus blazei polysaccharide was 75.1%,and ABP-A contained 88.6 GLc,2.4%Gal,1.9%Man,1.0%Rha,0.5%GalA,1.9%Xyl,2.1%Ara,and 1.6%Fuc.ABP-A significantly decreased liver index(P<0.05),and serum TC,TG,LDL-C(P<0.01),but significantly increased HDL-C(P<0.01),lipid droplets deposition in liver was decreased by ABP-A.LDL-R,SCARB-1F mRNA expression(P<0.05)and CYP7a-1,PPAR-αmRNA levels(P<0.01)significantly increased,and SREBP-1C mRNA expression dramatically decreased(P<0.01).These results indicated that ABP-A exerted significant effects on lowering blood lipid,and the mechanism may be related to regulate the expression of LDL-R,SREBP-1c,CYP7a-1,PPAR-αand SCARB-1F.
机构地区 北华大学药学院 中国人民解放军总医院血液科
出处 《食品科学技术学报》 CAS CSCD 北大核心 2021年第2期56-64,共9页 Journal of Food Science and Technology
基金 吉林省卫生技术创新项目(2017J083)。
关键词 姬松茸多糖 高脂血症 降血脂 固醇调节原件结合蛋白-1C 过氧化物酶体增殖物激活受体Α Agaricus blazei polysaccharide hyperlipidemia lipid lowering SREBP-1C PPAR-α
