摘要 基于巨噬-脂肪细胞共培养体系研究儿茶素对细胞炎性因子、炎性酶基因表达及AMPK、SIRT1磷酸化水平的影响,初步阐明儿茶素的抗炎作用机制。儿茶素的添加质量浓度为0,50,100μg/mL,采用定量PCR方法检测细胞炎性因子及炎性酶基因的表达水平,采用Western blotting方法检测AMPK及SIRT1的磷酸化水平。结果表明,各处理组中,儿茶素明显抑制促炎性因子(IL-1、IL-6和IL-12)及炎性酶(iNOS和COX-2)基因的表达,促进抗炎性因子(IL-4和IL-10)基因的表达,并且提高AMPK和SIRT1的磷酸化水平,AMPK和SIRT1两者之间呈协同作用。共培养体系中巨噬细胞TNF-α的分泌可诱导脂肪细胞的分化与成熟,儿茶素通过AMPK/SIRT1信号途径调节细胞炎性因子基因的表达实现其抗炎作用。 based on the macrophage and adipocytes co-cultured system,effect of catechins on the gene expression of cytokines and inflammatory enzymes,the phosphorylation levels of AMPK and SIRT1 was studied,and to preliminarily research the anti-inflammatory mechanism of catechin.Catechin with the mass concentrations of 0,50,100μg/mL were added to the four treatments,respectively.The gene expression of cytokines and inflammatory enzymes,phosphorylation levels of AMPK and SIRT1 in adipocytes were determined by quantitative RT-PCR and Western blotting,respectively.Catechin obviously inhibited the gene expression level of pro-inflammatory cytokines(IL-1,IL-6,IL-12)and inflammatory enzymes(iNOS,COX-2),but promoted the expression of anti-inflammatory cytokines(IL-4,IL-10).High mass concentration of catechin increased the phosphorylation level of AMPK and SIRT1,it is synergistic between AMPK and SIRT1.In the macrophage and adipocytes co-cultured system,the secretion of TNF-αin macrophages fully induced the differentiation of adipocytes.Catechin has an anti-inflammatory effect by regulating the gene expression of cytokines via AMPK/SIRT1 signaling pathway.
出处 《中国食品学报》 EI CAS CSCD 北大核心 2021年第4期64-70,共7页 Journal of Chinese Institute Of Food Science and Technology
基金 山东省农业重大应用技术创新项目(SD2019ZZ016) 湖南省教育厅专项(19A236) 山东省泰山学者人才工程项目(tshw20120747)。
分类号 R28 [医药卫生—中药学]
