摘要 以大菱鲆源温和气单胞菌群体感应调控基因luxI/luxR为研究对象,采用实时荧光定量PCR技术从转录水平上探究环境条件对该基因表达的影响,以期确定环境因素对群体感应系统的调节作用。结果表明:在适当低温(15℃)及弱碱(pH=8)环境条件下,luxI/luxR基因表达水平最高;而在高渗透压胁迫环境条件下,2种基因的表达水平明显下降。当葡萄糖为碳源时,luxI/luxR基因的表达量最高,且luxI基因对碳源的敏感程度高于luxR基因。不同氮源培养条件下luxI基因表达情况依次为胰蛋白胨>氯化铵>牛肉膏>酵母浸粉>脲素,而luxR基因表达情况依次为胰蛋白胨>氯化铵>酵母浸粉>牛肉膏>脲素。可以通过改变环境条件调节温和气单胞菌的群体感应系统,从而达到控制其致腐能力的目的。 Quorum sensing gene luxI/luxR of Aeromonas sobria was used for this study,and the effect of different environmental conditions on the expression of this gene was investigated from the transcription level by real-time fluorescence quantitative PCR to determine the regulatory effect of environmental factors on quorum sensing system.The results showed that the expression level of luxI/luxR gene was the highest at appropriate low temperature(15℃)and weak base(pH=8),while the expression level of both genes decreased under high osmotic stress.When glucose was the carbon source,the expression of luxI/luxR gene was the highest,and luxI gene was more sensitive to carbon sources than luxR gene.The expression level of luxI gene under different nitrogen sources was as follows:tryptone>ammonium chloride>beef extract>yeast extract>urea,and the expression of luxR gene was as follows:tryptone>ammonium chloride>yeast extract>beef extract>urea.Therefore,the changes of environmental conditions can be used to regulate quorum sensing system in Aeromonas sobria,so as to control its spoilage ability.
出处 《中国食品学报》 EI CAS CSCD 北大核心 2021年第4期71-78,共8页 Journal of Chinese Institute Of Food Science and Technology
基金 “十三五”国家重点研发计划重点专项(2018YFD 0400600,2017YFD0400106)。
分类号 O35 [理学—流体力学]
