摘要 目的:研究大豆分离蛋白与乳清蛋白混合形成的双蛋白对异基因造血干细胞移植小鼠造血及免疫重建的作用。方法:6~8周龄雄性BALB/c小鼠随机分为3组:正常对照组(CON)、造血干细胞移植组(HSCT+NP)、造血干细胞移植+双蛋白干预组(HSCT+PP)。造血干细胞移植后做4周蛋白干预实验,HSCT+PP组灌胃给予一定量的双蛋白悬液,HSCT+NP组给予等体积的生理盐水,CON组不做任何处理。每周测定各组小鼠血常规和小鼠体重,分别在移植后第2,4周处死小鼠,测定胸腺和脾脏指数,并在第4周测定各组小鼠骨髓造血干细胞含量及血清生化指标。结果:与蛋白未干预组(HSCT+NP)相比,双蛋白干预组(HSCT+PP)小鼠外周血白细胞、红细胞、血小板、淋巴细胞数量恢复显著加快(P<0.05),骨髓造血干细胞含量较多(P>0.05),胸腺指数显著增高(P<0.05),血清总蛋白(TP)、免疫球蛋白(IgG、IgA、IgM)含量较高,且IgM含量显著增高(P<0.05)。结论:造血干细胞移植术后补充双蛋白有利于加快小鼠的造血恢复和免疫功能重建。双蛋白营养支持和干预对造血干细胞移植术后患者造血重建和免疫功能恢复具有正向功效,有利于患者预后。 Objective:To study the effect of double protein powder formed by the combination of soy protein isolate and whey protein on hematopoiesis and immune reconstitution in mice after allogeneic hematopoietic stem cell transplantation.Methods:Male BALB/c mice aged 6-8 weeks were randomly divided into normal control group(CON),hematopoietic stem cell transplantation group(HSCT+NP),and hematopoietic stem cell transplantation+protein intervention group(HSCT+PP).Four weeks protein intervention experiment was performed after hematopoietic stem cell transplantation.HSCT+PP group was given a certain amount of double protein suspension,HSCT+NP group was given an equal volume of normal saline,and CON group without any treatment.The blood routine and mouse body weight of each group were measured weekly.The thymus and spleen indexes were measured at the 2nd and 4th week after transplantation,and the content of hematopoietic stem cells in bone marrow and serum biochemical parameters were determined at the time of the 4th week.Results:Compared with HSCT+NP group,the number of peripheral blood leukocytes,red blood cells,platelets and lymphocytes in the protein intervention group(HSCT+PP)recovered significantly faster(P<0.05),and the bone marrow hematopoietic stem cell content was higher(P>0.05),thymus index increased significantly(P<0.05),serum total protein(TP),immunoglobulin(IgG,IgA,IgM)content was higher,and IgM content was significantly increased(P<0.05).Conclusion:the supplementation of double protein after hematopoietic stem cell transplantation can accelerate blood cell recovery and immune function reconstruction in mice.Thus,the double protein can be used as a good protein nutritional supplement after hematopoietic stem cell transplantation,which is beneficial to the prognosis of patients.
机构地区 农业农村部食物与营养发展研究所
出处 《中国食品学报》 EI CAS CSCD 北大核心 2021年第4期122-130,共9页 Journal of Chinese Institute Of Food Science and Technology
基金 国家自然科学基金项目(31802306) 中国农业科学院科技创新工程项目(CAAS-ASTIP-2019-IFND)。
关键词 造血干细胞移植 双蛋白 造血 免疫重建 hematopoietic stem cell transplantation double protein hematopoiesis immune reconstitution
分类号 R73 [医药卫生—肿瘤]
