摘要 研究了大孔树脂分离纯化二角菱壳乙酸乙酯萃取液中多酚的方法。对7种大孔树脂进行了静态吸附和解吸的研究,筛选出了效果最佳的大孔树脂D101。对D101大孔树脂进行动态吸附和解吸条件的优化,最佳条件为:上样固形物质量浓度5mg/mL,上样流速1mL/min,洗脱溶剂乙醇体积分数70%,洗脱流速4mL/min,洗脱体积5 BV。总多酚含量由提取物冻干样品的478mg GAE/g上升到纯化后的825mg GAE/g,提高了1.73倍,回收率为59.43%。放大试验中,其回收率和纯化率并不随大孔树脂数量的增加而改变,说明该优化条件适合于大规模生产应用。 This study describes a method for separation and purification of polyphenolics of ethyl acetate extract(EAE)of Trapa bispinosa Roxb.pericarps(TBP)by macroporous resins.The abilities of static absorption and desorption of seven kinds of macroporous resins were studied.The best macroporous resin D101 was selected.The dynamic absorption and desorption conditions were optimized as follows:5 mg/mL of the sample concentration,1 mL/min of the flow rate of sample loading,70%of ethanol as the eluent,4 mL/min of the elution flow rate and5 BV of the elution volume.After enrichment by D101 macroporous resin,the content of total polyphenols increased from478 mg GAE/g in freeze dried extracts to 825 mg GAE/g in purified samples by 1.73 times,and the recovery was 59.43%.In the scale-up test,the recovery and purification rate did not change with the increase of macroporous resin,indicating that the optimized conditions were suitable for large-scale production.
出处 《食品与机械》 北大核心 2021年第4期47-52,共6页 Food and Machinery
基金 湖南省大学生创新创业训练计划项目(编号:20191636)。
关键词 二角菱 菱角壳 多酚 大孔树脂 吸附 解吸 Trapa bispinosa Roxb. pericarp polyphenols macroporous resin absorption desorption
分类号 R28 [医药卫生—中药学]
