摘要 为研究冷链流通过程中温度波动对水产品腐败菌生长代谢的影响,分析了3种不同温度波动的模拟冷链流通条件下腐败希瓦氏菌在凡纳滨对虾汁中的生长情况及其代谢产物挥发性盐基氮(total volatile basic nitrogen,TVB-N)、pH值、总氨基酸含量、生物胺含量的变化情况。结果表明,10℃条件下接种腐败希瓦氏菌的虾汁24 h内细菌快速增殖,而在4℃条件下经192 h后仍处于平稳增长趋势。温度波动在流通初期对腐败希瓦氏菌的生长影响较大,对中末期菌落数的影响较小。低温和温度波动并不影响腐败希瓦氏菌生长的最大菌落数,但会显著影响其生长速率。流通196 h后接菌组A1、B1、C1的TVB-N含量分别从初始的5.47 mg/100 g上升至28.29、13.11和25.91 mg/100 g,表明温度波动显著提高腐败产物的累积。接菌组样品的总氨基酸含量均呈下降趋势,且以赖氨酸、酪氨酸和精氨酸下降最为明显。腐败希瓦氏菌产生的生物胺主要是腐胺和尸胺。综上所述,贮运流通初期发生温度波动对腐败希瓦氏菌的生长影响较大,会加速氨基酸的降解和腐败产物的生成。 Effect of fluctuating temperatures on the growth of spoilage bacteria and quality changes of aquatic products was investigated during simulated cold logistic processes.The growth of Shewanella putrefaciens in Pacific white shrimp juice were studied and the changes of total volatile basic nitrogen(TVB-N),pH,total amino acid,and biogenic amine in the shrimp juice were analyzed.The results showed that S.putrefaciens proliferated within 24 h at 10℃,while grew gradually within 192 h at 4℃.Temperature fluctuation accelerated the growth of S.putrefaciens at the early transportation period but had little effect at the mid and later period.Low temperature and temperature fluctuation did not inhibit the maximum bacterial counts but affected the growth rate.After 196 h of storage,the TVB-N values of inoculated samples A1,B1 and C1 increased from 5.47 mg/100 g to 28.29 mg/100 g,13.11 mg/100 g,and 25.91 mg/100 g respectively,indicating that temperature fluctuation accelerated the accumulation of spoilage products.The total amino acids of inoculated samples all decreased,especially lysine,tyrosine,and arginine.The most important biogenic amines produced by S.putrefaciens were putrescine and cadaverine,and their changes were similar with TVB-N.Therefore,temperature fluctuation at the early period played an important role in the growth and metabolic activity of S.putrefaciens,which accelerated the degradation of amino acids and production of spoilage compounds.
出处 《食品与发酵工业》 CAS CSCD 北大核心 2021年第9期100-106,共7页 Food and Fermentation Industries
基金 国家自然科学基金青年基金项目(31501551) 上海海洋大学博士科研启动基金项目(A2-2006-00-200360) 上海海洋大学专项科研基金项目(A2-2006-20-200203)。
关键词 凡纳滨对虾 腐败希瓦氏菌 温度波动 氨基酸 生物胺 致腐败 Litopenaeus vannamei Shewanella putrefaciens temperature fluctuation amino acid biogenic amines spoilage potential
