摘要 草莓叶水提物(water extractions from strawberry leaves,SLWE)富含多种生物活性成分,能促进成纤维细胞(fibroblasts,FB)合成胶原蛋白,而胶原蛋白只有经过骨形态发生蛋白1(bone morphogenetic protein 1,BMP-1)的剪切才能变成有活性的蛋白。该文通过对SLWE主成分分析及主要酶活力测定,同时探究SLWE对人包皮成纤维细胞(human foreskin fibroblasts,HFF-1)合成胶原蛋白I(collagen I,COL I)及分泌BMP-1的影响。结果表明:SLWE中维生素C、原花青素、多糖、总黄酮、总酚、蛋白质的含量分别为(0.88±0.025)、(0.28±0.027)、(10.00±0.278)、(1.47±0.052)、(2.05±0.031)、(0.10±0.003)mg/mL;超氧化物歧化酶、过氧化物酶的酶活力分别为125.59、0.06 U/mL;SLWE对HFF-1细胞增殖作用不明显,但具有促进其COL I表达和增加BMP-1分泌量的作用。实验结果表明SLWE含有丰富的活性成分,具有促进HFF-1中COL I分泌和成熟的作用,且此功效并非通过增加细胞数量而实现的。综上所述,SLWE具备作为延缓衰老化妆品的功效成分的潜能,为草莓叶等废弃物资源再利用提供了理论依据。 Water extractions from strawberry leaves(SLWE)contains a variety of bioactive components,which can stimulate fibroblasts to synthesize collagens.And collagens must be proteolytic cleavaged by bone morphogenetic protein 1(BMP-1)to turn into an active form.In this study,the bioactive component contents and main antioxidant enzyme activities of SLWE were analyzed.Furthermore,the effect of SLWE on collagen I(COL I)synthesis and BMP-1 secretion by human foreskin fibroblasts(HFF-1)was evaluated.The results showed that the contents of Vc,proanthocyanidins,polysaccharides,total flavonoids,total phenols and protein in SLWE were(0.88±0.025),(0.28±0.027),(10.00±0.278),(0.47±0.031),(0.10±0.003)mg/mL,respectively.SLWE also presented antioxidant activity with 125.59 U/mL,0.06 U/mL for SOD and POD,respectively.Though SLWE had no obvious effect on the proliferation of HFF-1 cells,it promoted COL I synthesis and secretion of BMP-1.Overall,our study indicated that SLWE contained abundant bioactive components and promoted the secretion and maturation of collagens in HFF-1 without increasing the cell number of HFF-1.Therefore,SLWE has been demonstrated as a potential functional ingredient for anti-aging cosmetics.Our research also provides theoretical basis for the reuse of agricultural wastes like strawberry leaves.
出处 《食品与发酵工业》 CAS CSCD 北大核心 2021年第9期114-119,共6页 Food and Fermentation Industries
基金 北京市科技计划资助项目(Z181100002418014) 北京市教委科研计划一般资助项目(KM201810011007)。
关键词 草莓叶水提物 人包皮成纤维细胞 胶原蛋白I 骨形态发生蛋白1 延缓衰老 water extractions from strawberry leaves human foreskin fibroblasts collagen I bone morphogenetic protein 1 anti-aging
分类号 R28 [医药卫生—中药学]
