摘要 为探究鸡肉制品在加工过程中金黄色葡萄球菌的肠毒素基因分布、分子分型和耐药性情况,本研究对某鸡肉制品加工厂的生产加工过程进行取样,通过DNA提取、PCR扩增nuc基因对金黄色葡萄球菌进行分离鉴定,然后分析肠毒素基因分布情况,对获得带有毒素基因的菌株进行多位点序列分型(MLST)和耐药性研究。结果表明,260份样本中分离到38株金黄色葡萄球菌(其中携带肠毒素基因的菌株有24株),检出率为63.16%;共检测到7种毒素基因型,其中sed携带率最高(60.53%),依次为seg(26.32%)、see(21.05%)、sei(18.42%)、sec(10.53%)、sea(7.89%)、seh(5.26%),携带两种及以上肠毒素基因的菌株有14株(36.84%)。24株携带毒素基因的菌株MLST分型都为ST型,分为3种ST分型,16株为ST7序列型(66.67%);5株为ST5序列型(20.83%);3株为ST464序列型(12.5%)。耐药性分析实验结果表明,抑菌效果最好的抗生素是万古霉素,38株菌株都对其敏感(100%);绝大多数菌株都对抗生素有耐药性,依次为青霉素G(86.84%)、环丙沙星(60.53%)、克林霉素(55.26%)、四环素(52.63%);对3-6种抗生素耐药的金黄色葡萄球菌菌株分别占18.42%、15.79%、23.68%、7.89%。研究结果表明鸡肉产品中存在金黄色葡萄球菌污染的情况,并且其中存在携带多种类型肠毒素基因的菌株,也存在具有多重耐药性的菌株。 In order to explore the enterotoxin gene distribution,molecular typing and drug resistance of Staphylococcus aureus during the processing of chicken products,samples were taken from the production and processing process of a chicken products processing factory.The Staphylococcus aureus was isolated and identified by DNA extraction and PCR amplification of nuc gene,and then the distribution of enterotoxin gene was analyzed to obtain the bacteria with toxin gene multi locus sequence typing(MLST)and drug resistance were studied.Results showed that,38 strains of Staphylococcus aureus were isolated from 260 samples(including 24 strains carrying enterotoxin gene),and the detection rate was 63.16%.A total of 7 enterotoxin genotypes were detected,with the highest sed carrying rate 60.53%,followed by seg(26.32%),see(21.05%),sei(18.42%),sec(10.53%),sea(7.89%),and seh(5.26%).Among these 24 strains carrying the enterotoxin gene,there was 14 strains(36.84%)carrying two or more enterotoxin genes.And these 24 strains were divided into 3 ST genotypes of the MLST,including ST7(66.67%),ST5(20.83%)and ST464(12.5%).The resistance against antibiotics of the 38 isolates showed that the strains were sensitive to vancomycin(100%),and more than half of the strains were resistant to other antibiotics,such as penicillin G(86.84%),ciprofloxacin(60.53%),clindamycin(55.26%),and tetracycline(52.63%).The multi-drug resistance rate of the strain reached 65.78%,among which Staphylococcus aureus strains resistant to 3 antibiotics accounted for 18.42%,resistant to 4 antibiotics accounted for 15.79%,resistant to 5 antibiotics accounted for 23.68%,and resistant to 6 antibiotics accounted for 7.89%.These results indicated that there were contamination of Staphylococcus aureus in chicken processing,and the strains carried multiple types of enterotoxin genes,as well as multiple drug resistance.
机构地区 西北农林科技大学食品科学与工程学院
出处 《食品工业科技》 CAS 北大核心 2021年第10期68-74,共7页 Science and Technology of Food Industry
基金 国家重点研发计划项目(2018YFC1602201) 国家自然基金项目(31471638) 浙江省公益性技术应用研究(分析测试)项目(2018C37015) 质检总局科技计划项目(2017IK206) 西北农林科技大学大学生科技创新项目(X201910712070)。
关键词 鸡肉制品 金黄色葡萄球菌 肠毒素基因 MLST分型 耐药性 chicken processing Staphylococcus aureus enterotoxin gene multi-site sequence typing(MLST) resistance against antibiotic
