摘要 利用高通量测序技术研究成品陶融型大曲的微生物群落结构,并与大曲的理化性质进行相关性分析。结果表明:大曲中共检测到83个细菌可操作分类单元(operational taxonomic units,OTU),主要细菌类群为厚壁菌门(Firmicutes)占79.15%、变形菌门(Proteobacteria)占15.30%、放线菌门(Actinobacteria)占4.05%、蓝藻门(Cyanobacteria)占1.42%,其他占0.08%,优势菌属为乳杆菌属(Lactobacillus)、魏斯氏菌属(Weissella)、泛菌属(Pantoea)、明串珠菌属(Leuconostoc)、Kroppenstedtia、糖多孢菌属(Saccharopolyspora)、片球菌属(Pediococcus)、芽孢杆菌属(Bacillus)、Enterobacteriaceae_unclassified、Mitochondria_norank、伯克霍尔德菌属(Burkholderia)、Cyanobacteria_norank;检测到34个真菌OTU,其中子囊菌门(Ascomycota)为绝对优势菌群,占99.19%,优势菌属为unidentified、嗜热真菌属(Thermomyces)、嗜热子囊菌属(Thermoascus)、曲霉属(Aspergillus)、根毛霉属(Rhizomucor)、威克汉姆酵母(Wickerhamomyces)、哈萨克斯坦酵母(Kazachstania);陶融型大曲中细菌的多样性远高于真菌,大曲主体微生物明显,不同种属的微生物与大曲相关理化指标呈显著相关性。该研究对于指导陶融型大曲制作工艺的优化及陶融型白酒的生产具有重要意义。 The microbial community structure in mature Taorong-type Daqu was studied by high-throughput sequencing,and its correlation with the physicochemical properties of Daqu was analyzed.The results showed that a total of 83 bacterial operational taxonomic units(OTUs)were detected in Daqu.The main bacterial phyla included Firmicutes(79.15%),Proteobacteria(15.30%),Actinobacteria(4.05%),Cyanobacteria(1.42%),and others(0.08%).The dominant genera were Lactobacillus,Weissella,Pantoea,Leuconostoc,Kroppenstedtia,Saccharopolyspora,Pediococcus,Bacillus,Enterobacteriaceae_unclassified,Mitochondria_norank,Burkholderia and Cyanobacteria_norank.A total of 34 fungal OTUs were detected,among them,Ascomycota was absolutely dominant(99.19%).The dominant fungi were unidentified,Thermomyces,Thermoascus,Aspergillus,Rhizomucor,Wickerhamomyces and Kazachstania.The bacterial community in Taorong-type Daqu was much more diverse than the fungal community.The major microorganisms were predominant,and all identified microbial genera were significantly correlated with the physicochemical properties of Daqu.This study will be of great significance to guide the optimization of the production of Taorong-type Daqu and liquor.
出处 《食品科学》 EI CAS CSCD 北大核心 2021年第8期106-113,共8页 Food Science
基金 国家自然科学基金青年科学基金项目(31801535) 河南省重大科技专项(181100211400) 中国轻工业浓香型白酒固态发酵重点实验室开放基金项目(2018JJ017) 酿酒生物技术及应用四川省重点实验室开放基金项目(NJ2019-04)。
关键词 陶融型大曲 微生物群落 高通量测序 相关性 Taorong-type Daqu microbial community high-throughput sequencing correlation
