摘要 【目的】果胶是植物细胞壁的主要成分和结构多糖。果胶甲酯酶(PME)是一种重要的果胶修饰酶,具有调节细胞壁弹性和通透性的作用,在植物的生长发育过程中发挥作用。本研究探讨枣果胶甲酯酶基因家族的基本信息和特点,为进一步阐明ZjPMEs基因的功能、筛选果实质地密切相关基因奠定基础。【方法】本研究基于‘冬枣’基因组,鉴定枣基因组中的果胶甲酯酶基因;基于RNA-Seq数据,分析枣果胶甲酯酶基因家族的特征以及表达模式。【结果】共鉴定出46个枣果胶甲酯酶基因,不均匀分布在10条染色体上。系统演化分析表明这些基因包含29个Type-Ⅰ型和17个Type-Ⅱ型成员,进一步可以划分为4个亚家族。在每个亚家族中,基因共享保守的基因结构和基序组成。基因表达分析显示该家族基因在花中表达丰度较高,可能在枣的生殖发育过程起重要作用。通过分析该家族基因在不同质地的‘冬枣’(酥脆)和‘P15’(硬质)以及在不同用途的‘冬枣’(鲜食)和‘骏枣’(制干)果实发育过程中的表达模式,筛选出与枣果实质地关系密切的基因ZjPME 18,该基因在‘P15’和‘骏枣’果实中表达量较高。【结论】枣果胶甲酯酶基因家族具有特定的结构特征及表达模式,ZjPME 18的表达与调控可能与枣果实质地密切相关。 [Objective]Pectin is a major component and structural polysaccharide of the cell walls of plants.Pectin methylesterase(PME)is a key enzyme that modifies pectin,and thereby regulates cell wall rigidity and elasticity,playing a significant role in the plant development.The purpose of this study was to investigate the basic information and characteristics of ZjPMEs,which could serve as a foundation for further elucidation of the functions of ZjPME genes and screening of genes closely related to fruit texture.[Method]We identified the family genes in the‘Dongzao’genome using bioinformatics softwares.The characteristics and expression patterns of the genes were analyzed based on RNA-seq data.[Result]46 PME genes were identified in the jujube genome,including 29 Type-ⅠZjPMEs and 17 Type-ⅡZjPMEs,which were classified into four subfamilies(Ⅰ-Ⅳ)and unevenly distributed across 10 chromosomes.Within each subfamily,the genes shared conserved gene structure and motif compositions.expression analysis revealed that most of the genes were expressed highly in flowers,indicating the important function of PME genes in the reproductive developmental process.Furthermore,by analyzing the expression patterns of the ZjPMEs during the fruit development between‘Dongzao’(crisp)and‘P15’(firm),and between‘Dongzao’(consumed freshly)and‘Junzao’(consumed as dry fruit),we found ZjPME18 might be closely related to fruit texture.ZjPME18 showed higher expression in‘Junzao’and‘P15’.[Conclusion]The ZjPMEs have specific structure characteristics and expression patterns,and the expression and regulation of ZjPME18 may be closely related to the fruit texture.
出处 《北京林业大学学报》 CAS CSCD 北大核心 2021年第4期8-16,共9页 Journal of Beijing Forestry University
基金 国家科技计划重大专项课题(2018YFD1000607)。
