摘要 背景与目的:已有研究显示,DEP结构域蛋白质1B(DEPDC1B)在多种肿瘤中异常表达并在肿瘤进展中发挥重要作用,DEPDC1B在胰腺癌中的表达水平及临床意义尚不清楚。本研究通过检测胰腺癌组织中DEPDC1B的表达,分析其与胰腺癌患者临床病理特征及预后的关系,从而探讨其在胰腺癌中的临床意义。方法:下载癌症基因组图谱(TCGA)和高通量基因表达数据库(GEO)的胰腺癌患者临床数据,分析DEPDC1B在胰腺癌组织中的表达情况,采用Kaplan-Meier生存曲线及Cox风险模型分析DEPDC1B表达与胰腺癌患者预后的关系。采用免疫组织化学法检测97例胰腺癌组织和癌旁组织中DEPDC1B的表达,根据患者临床资料进一步分析DEPDC1B与胰腺癌临床病理特征以及胰腺癌患者预后的关系。结果:在TCGA与GEO的GSE16515、GSE15471和GSE28735数据集中,DEPDC1B在胰腺癌组织中的表达明显高于匹配的正常组织(均P<0.05);在TCGA数据库与GSE28735数据集中,DEPDC1B高表达胰腺癌患者生存时间明显缩短(均P<0.05);TCGA中DEPDC1B表达是胰腺癌患者预后的独立危险因素(HR=1.32,95%CI=1.118~1.559,P=0.001)。97例临床胰腺癌患者中,胰腺癌组织中DEPDC1B的阳性表达率明显高于癌旁组织胰腺组织(69.07%vs.11.34%,P<0.001);DEPDC1B的表达与分化程度、临床分期及淋巴结转移有关(均P<0.05);DEPDC1B高表达患者总生存期明显短于低表达患者(P<0.05);DEPDC1B表达是预后的独立危险因素(HR=1.126,95%CI=1.012~1.253,P=0.029)。结论:DEPDC1B高表达可能参与胰腺癌的发生、发展及转移过程,并可作为判断胰腺癌预后的重要指标。 Background and Aims:Previous studies have shown that DEP domain protein 1B(DEPDC1B)is abnormally expressed in a variety of tumors and plays an important role in tumor progression.However,the expression level and clinical significance of DEPDC1B in pancreatic cancer are still unclear.Therefore,this study was aimed to investigate the clinical significance of DEPDC1B in pancreatic cancer by determining the DEPDC1B expression in pancreatic cancer tissue and its association with the clinicopathologic parameters and prognosis of pancreatic cancer patients.Methods:The clinical data of pancreatic cancer patients were downloaded from The Cancer Genome Atlas(TCGA)and Gene expression Omnibus(GEO)databases,and then,the DEPDC1B expressions in pancreatic cancer tissue were analyzed,and the relation of DEPDC1B expression with the prognosis of pancreatic cancer patients was determined by Kaplan-Meier survival curve and Cox risk model.The DEPDC1B expression in 97 paired specimens of pancreatic cancer and adjacent normal tissues was detected by immunohistochemical staining,and the associations of DEPDC1B expression with clinicopathologic variables and prognosis were evaluated.Results:The DEPDC1B expression of in pancreatic cancer tissue was significantly higher than that in matched normal tissues in TCGA database and the GSE16515,GSE15471 and GSE28735 datasets of GEO database(all P<0.05);the survival time of pancreatic cancer patients with high DEPDC1B expression was significantly shortened in TCGA database and GSE28735 dataset;DEPDC1B expression was an independent risk factor for the prognosis of pancreatic cancer patients in TCGA database(HR=1.32,95%CI=1.118–1.559,P=0.001).In the 97 clinical pancreatic cancer patients,the positive DEPDC1B expression rate was significantly higher in pancreatic cancer tissue than that normal tissue(69.07%vs.11.34%,P<0.001);the DEPDC1B expression was significantly associated with the degree of differentiation,clinical stage and lymph node metastasis(all P<0.05);the overall survival of patients wi
出处 《中国普通外科杂志》 CAS CSCD 北大核心 2021年第3期261-268,共8页 Chinese Journal of General Surgery
基金 国家自然科学基金资助项目(81900772) 江苏省连云港市卫生计生科技资助项目(201802,201906) 南京医科大学康达学院第一附属医院博士科研启动基金资助项目(BS202003)。
关键词 胰腺肿瘤 DEP结构域蛋白质1B 预后 生物标记 肿瘤 Pancreatic Neoplasms DEP Domain Protein 1B Prognosis Biomarkers,Tumor
分类号 R735.9 [医药卫生—肿瘤]
