摘要 目的建立基于传统中药"辨状论质"的厚朴饮片质量综合评价指数(Qi),利用Qi值高低评价其质量,划分饮片等级,并采用药效学方法评价药效强弱。方法对传统优质厚朴"皮厚、肉紫、气辛"的评价进行定量化描述,分别测定57批厚朴饮片的平均厚度、粉末颜色、挥发油成分β-桉叶醇以及厚朴酚、和厚朴酚和辣薄荷基厚朴酚的含量,以三角形面积法建立厚朴饮片Qi,根据Qi值划分饮片等级,并采用硫酸阿托品引起的小鼠胃排空和小肠推进抑制模型,评价不同质量样品药效强弱。结果所有样品Qi值范围为0.17~0.87,说明饮片质量差异明显。以Qi值划分饮片等级,一等样品Qi值≥0.47,二等样品Qi值在0.35~0.47,三等样品Qi值<0.35。药效结果显示,饮片Qi值与胃内残留率呈现显著的负相关,说明胃排空作用与饮片Qi值呈正相关,饮片Qi值高,饮片质量优质,药效作用强。结论首次在传统"辨状论质"基础上利用Qi值对厚朴饮片进行等级划分,并选择与其"下气除满"功效相关的药理模型,揭示了Qi值和药效强弱的相关性,为中药饮片等级评价提供了新的思路。 Objective To establish a comprehensive quality evaluation index(Qi)for the Magnolia officinalis decoction pieces based on the"quality evaluation through morphological identification".Classification of these decoction pieces was performed based on Qi value and pharmacodynamic methods was carried out to evaluate their efficacy.Methods The traits of M.officinalis"thick bark,purple endocuticle and pungent flavor"was quantitatively described,and the average thickness,powder color,the contents ofβ-eudesmol,honokiol,magnolol and piperitylmagnolol of 57 batches samples were measured.The quality comprehensive evaluation index(Qi)was established by the triangle area method,the decoction pieces were graded according to the Qi value,and the inhibition of gastric emptying and intestine propulsion in mice model was induced by atropine sulfate and the potency of different grade samples was evaluated.Results The Qi value was ranged from 0.17 to 0.87,indicating that the quality of decoction pieces was significantly different.The Qi value is used to classify the decoction pieces,the first-class sample with Qi≥0.47,the second-class sample with a Qi value was from 0.35 to 0.47,and the Qi value<0.35 as the third-class sample.The efficacy results showed that the Qi value was significantly negatively correlated with the residual rate in the stomach,demonstrating that the gastric emptying effect was positively correlated with the Qi value.Consequently,the Qi value of the decoction pieces was high,indicating the quality and he effect of the decoction pieces was higher and stronger.Conclusion based on the"Quality evaluation through morphological identification",the classification M.officinalis decoction pieces is firstly reported by a comprehensive evaluation index(Qi).It reveals the positive correlation between the potency of the drug and the Qi value and provides a new idea for the grade evaluation of Chinese herbal medicine.
机构地区 中国食品药品检定研究院 中国中医科学院中药研究所
出处 《中草药》 CAS CSCD 北大核心 2021年第8期2285-2293,共9页 Chinese Traditional and Herbal Drugs
基金 国家中药标准化项目(ZYBZH-Y-ZY-45)。
关键词 厚朴饮片 含量测定 外观性状 综合评价指数 等级划分 药效 Magnolia officinalis decoction pieces content determination appearance traits comprehensive evaluation index grade division efficacy
