摘要 目的探究黄绵马酸BB对3种革兰阳性致病菌的抗菌活性及生物被膜的清除作用。方法采用微量稀释法测定黄绵马酸BB对耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)、粪肠球菌(Enterococcus faecalis,EFA)及屎肠球菌(Enterococcus faecium,EFM) 15株临床分离株的最小抑菌浓度(minimum inhibitory concentration,MIC)和最小杀菌浓度(minimum bactericidal concentration,MBC);考察黄绵马酸BB对MRSA2、EFA13和EFM15生长周期的影响;采用CCK-8法、扫描电子显微镜(SEM)考察黄绵马酸BB对MRSA2、EFA13和EFM15生物被膜的清除作用。结果黄绵马酸BB对MRSA的MIC为11.49~63.50μg/mL、MBC为22.98~160.00μg/mL,对EFA的MIC为7.07~160.00μg/mL、MBC为28.28~160.00μg/mL,对EFM的MIC为16.82μg/mL、MBC为56.57μg/mL,其中黄绵马酸BB对MRSA1、2和EFA14、15的MIC均小于莫匹罗星;黄绵马酸BB对MRSA2、EFA13和EFM15的生长具有抑制作用,能延迟受试菌进入对数生长期;MIC黄绵马酸BB对MRSA2、EFA13和EFM15生物被膜的清除率分别达到85.15%、76.48%、82.31%,均优于莫匹罗星、万古霉素、达托霉素;黄绵马酸BB对MRSA2生物被膜聚集及成熟阶段的清除效果优于达托霉素,对EFA13生物被膜聚集及成熟阶段的清除效果优于莫匹罗星、万古霉素、达托霉素,对EFM15生物被膜成熟阶段的清除效果优于莫匹罗星、万古霉素、达托霉素。结论黄绵马酸BB具有良好的体外抑菌活性以及对生物被膜的清除作用。 Objective To explore the antibacterial activity of flavaspidic acid BB on three kinds of gram-positive pathogenic bacteria and the scavenging effect of biofilm. Methods Microdilution method was used to determine the minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) of flavaspidic acid BB on 15 clinical isolates of methicillin-resistant Staphylococcus aureus(MRSA), Enterococcus faecalis(EFA) and Enterococcus faecium(EFM);Effect of flavaspidic acid BB on growth cycle of MRSA2, EFA13 and EFM15 was investigated;CCK-8 method and scanning electron microscopy(SEM) were used to investigate the scavenging effect of flavaspidic acid BB on MRSA2, EFA13 and EFM15 biofilms. Results MIC and MBC of flavaspidic acid BB for MRSA were 11.49 — 63.50 μg/mL and 22.98 — 160.00 μg/mL;MIC and MBC for EFA were 7.07 — 160.00 μg/mL and 28.28 — 160.00 μg/mL;MIC and MBC for EFM were 16.82 μg/mL and 56.57 μg/mL. MIC of flavaspidic acid BB for MRSA1 — 2 and EFA14 — 15 were lower than that of mupirocin. Growth of MRSA2, EFA13 and EFM15 were inhibited by flavaspidic acid BB, which could delay the test bacteria entering the logarithmic growth phase. Clearance rates of MRSA2, EFA13 and EFM15 biofilms by flavaspidic acid BB(MIC) were respectively 85.15%, 76.48% and 82.31%, which were better than mupirocin, vancomycin and daptomycin. Clearing effect of flavaspidic acid BB on MRSA2 biofilm in accumulation and maturation stage were better than daptomycin, clearing effect of flavaspidic acid BB on EFA13 biofilm in accumulation and maturation stage were better than mupirocin, vancomycin and daptomycin, clearing effect of flavaspidic acid BB on EFM15 biofilm in maturation stage were better than mupirocin, vancomycin and daptomycin. Conclusion Flavaspidic acid BB had a good antibacterial activity in vitro and clearing effect on biofilms.
出处 《中草药》 CAS CSCD 北大核心 2021年第8期2324-2330,共7页 Chinese Traditional and Herbal Drugs
基金 国家重点研发计划“中医药现代化”重点专项项目(2018YFC1707100) 广东省科技厅应用型研发专项(2015B020234009)。
关键词 革兰阳性球菌 黄绵马酸BB 抗菌药物 抗菌活性 生物被膜 gram-positive bacteria flavaspidic acid BB antibacterial agents antibacterial activity biofilm
